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GAPDH Loading Control Antibody (GA1R)
 
   
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GAPDH Loading Control Antibody (GA1R)

该抗体单克隆抗体,克隆号为GA1R。其抗原为纯化的猪来源GAPDH蛋白,其检测种属非常的广泛,可识别人、牛、猪、小鼠、大鼠等其它哺乳动物,以及鸟类的GAPDH蛋白,是一个真正保守的内参抗体。 该β-Tubulin内参抗体免疫荧光的效果也非常棒,是一款非常适合做免疫荧光对细胞骨架进行染色定位的抗体产品。另外,也有客户报告使用该抗体用于组织的荧光染色,也取得了意外的效果

  • 商品编号:MA5-15738
  • 货  号:MA5-15738
  • 品  牌:友乐博
  • 计量单位:100 µg
  • 所得积分:1453
  • 市场价: ¥1896.00
  • 销售价: ¥1580.00
  • 节省: ¥316.00
购买数量:
  (库存9999+)

    在Western Bloting实验中,可能存在总蛋白浓度测定不准确,或者蛋白质样品在电泳前上样时产生的样品间的操作误差;这些误差可以通过测定每个样品中实际转到膜上的内参,比如内参GAPDH的含量来进行校正。GAPDH(甘油醛-3-磷酸脱氢酶)是参与糖酵解的一种关键酶,由4个30-40kDa的亚基组成,分子量146kDa,检测条带大约在36kDa。 GAPDH基因几乎在所有组织中都高水平表达,广泛用作Western blot蛋白质标准化的内参。

    内参抗体虽然选择较多,但是也需要遵循一定的原则,并契合实际的应用要求。首先,我们需要考虑目的蛋白的大小,我们推荐内参要与检测的目的蛋白的分 子量最好相差5KD以上,因此你需要根据你目的检测蛋白的分子量来选择合适的内参,由于GAPDH的检测分子量大约在36kD,因此该内参抗体不是很适合用于检测30kD-40kD大小目标蛋白的WB实验中。另外,不同的组织来源的样本在选择内参时还有一定的区别。比如某些细胞中,由于组织缺氧、糖尿病等因素会导致GAPDH的表达增高,不适合做内参。

GAPDH Loading Control Antibody (GA1R),该抗体单克隆抗体,克隆号为GA1R。其抗原为纯化的猪来源GAPDH蛋白,其检测种属非常的广泛,可识别人、牛、猪、小鼠、大鼠等其它哺乳动物,以及鸟类的GAPDH蛋白,也算是一个真正保守的内参抗体。另外,该抗体的效价非常高,WB实验推荐稀释比率为 1:1000-1:10000,甚至有客户报告更高的稀释比率。

该β-Tubulin内参抗体免疫荧光的效果也非常棒,是一款非常适合做免疫荧光对细胞骨架进行染色定位的抗体产品。另外,也有客户报告使用该抗体用于组织的荧光染色,也取得了意外的效果。

除了100μl小包装,友乐博还提供大包装500ug的GAPDH内参抗体,非常适合经常进行内参验证或校准,或者进行细胞骨架荧 光定位的免疫学实验需求。

GAPDH Loading Control Antibody (GA1R)

GAPDH Loading Control Monoclonal Antibody for ELISA, IF, Western blot

Details
Host / Isotype:Mouse / IgG1
Class:Monoclonal
Type:Antibody
Clone:GA1R
Species Reactivity:Yeast (Ys) Bacteria (Ba) Insect (Ins) Human (Hu) Murine (Ms) Rat (Rt) Rabbit (Rb) Hamster (Hm) Chicken (Ck)
Immunogen:Recombinant GAPDH


ApplicationsDilution *
Western Blot (WB)1:1000 - 1:10,000
Immunofluorescence (IF)1:500 - 1:2000
ELISA (ELISA)Assay Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.


Product Specific Information
MA5-15738 detects GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, hamster, and chicken samples. 

MA5-15738 has been successfully used in Western blot, ELISA, and immunofluorescence. 

MA5-15738 may be stored at 4ºC short term. Add 0.05% sodium azide if desired. For long term storage, store at -20ºC, avoiding freeze/thaw cycles.

Ordering Information
Storage:Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form:100 µg Protein A purified antibody (1 mg/ml) in 10 mM PBS, pH 7.2.

General Information
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. In addition to this role, it is involved in other cellular processes ranging from membrane fusion, neuronal apoptosis to cancer. GAPDH is expressed at high levels in most tissues and is therefore useful as protein loading control in western blot analysis. Additionally, the antigen is conserved in most eukaryotes.



Product Images

·         GAPDH Loading Control Antibody (MA5-15738) in WB

Western Blot with anti-GAPDH Loading Control Monoclonal Antibody [GA1R] (MA5-15738)

Western blot analysis of GAPDH was performed by loading 20ug of THP-1 whole cell lysates per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% milk in TBST. The membrane was probed with a GAPDH loading control monoclonal antibody (Product # MA5-15738) at a dilution of 1:5000 overnight at 4ºC, washed in TBST, and probed with a HRP-conjugated goat anti-mouse IgG at a dilution of 1:40,000 for 1 hr at room temperature. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.

·         GAPDH Loading Control Antibody (MA5-15738) in WB

Western Blot with anti-GAPDH Loading Control Monoclonal Antibody [GA1R] (MA5-15738)

1:2000 (0.5ug/mL) Ab dilution used in WB of 5ug/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6).

 

·         GAPDH Loading Control Antibody (MA5-15738) in WB

Western Blot with anti-GAPDH Loading Control Monoclonal Antibody [GA1R] (MA5-15738)

WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3)

 

 

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