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6x-His Epitope Tag Antibody (HIS.H8)
 
   
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6x-His Epitope Tag Antibody (HIS.H8)

His标签抗体, 抗His单抗,His小鼠源单克隆抗体 His标签是由6个组氨酸(His-His-His-His-His-His)组成的短肽,专门设计用于重组蛋白质的吸附纯化。由于分子量较小,并且较容易分离和纯化,His融合标签与其他标签相比有很多明显优势, 是目前用于纯化的融合标签中使用最为广泛的一种。His抗体可以用于检测和His标签融合表达蛋白的表达、细胞内定位,以及纯化、定性或定量检测His融合表达蛋白等。

  • 商品编号:MA1-21315
  • 货  号:MA1-21315
  • 品  牌:友乐博
  • 计量单位:100ul
  • 所得积分:1702
  • 市场价: ¥2220.00
  • 销售价: ¥1850.00
  • 节省: ¥370.00
购买数量:
  (库存9999+)
    His标签是由6个组氨酸(His-His-His-His-His-His)组成的短肽,专门设计用于重组蛋白质的吸附纯化。由于分子量较小,并且较容易分离和纯化,His-tag 融合标签与其他标签相比有很多明显优势, 是目前用于纯化的融合标签中使用最为广泛的一种。His抗体可以用于检测和His标签融合表达蛋白的表达、细胞内定位,以及纯化、定性或定量检测His融合表达蛋白等。然而,目前商品化His-tag的单克隆抗体(mAb)种类不多, 且价格也十分昂贵。
    我们在选择His抗体时,主要根据自己的需要,特别是实验应用上的需要。其中需要考虑的因素包括了,His抗体的应用检测类型、His抗体的克隆性及制备来源等。比如该His抗体是否能用于Western Bloting的检测?是否可以用于免疫荧光IF实验?我是否需要特异性更好的单克隆His标签抗体?我是否需要考虑下是选择小鼠来源的还是兔来源的His抗体?
    本产品6x-His Epitope Tag Antibody (HIS.H8)抗体与Abcam抗体来源于同一种克隆细胞系,均为HIS.H8。与Abcam一样,其免疫原是6x His合成多肽,可识别可识别His重组蛋白和在细胞内过表达的His标记重组蛋白。然而其价格比Abcam低65%以上。该抗体可以用于Western Bloting、免疫细胞化学及免疫荧光ICC/IF和免疫沉淀IP实验等多种不同应用,适合经常进行His标签系统免疫学检测的实验需求
    该抗体在免疫细胞化学及免疫荧光ICC/IF中表现非常优秀,HIS.H8克隆的His标签抗体识别的免疫决定簇非常适合ICC/IF的应用。对IP免疫沉淀反应也很适合。


6x-His Epitope Tag Antibody (HIS.H8)

6x-His Epitope Tag Monoclonal Antibody for DB, ELISA, IF, IP, Western blot

Details

Host / Isotype:

Mouse / IgG2b

Class:

Monoclonal

Type:

Antibody

Clone:

HIS.H8

Species Reactivity:

Tag (Tag)

Immunogen:

6x His synthetic peptide.

Applications

Dilution *

ELISA (ELISA)

Assay dependent

Immunoprecipitation (IP)

Assay dependent

Western Blot (WB)

1:1000 - 1:3000

Immunofluorescence (IF)

1:500 - 1:2000

Dot blot (DB)

Assay dependent

* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

General Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Expression of recombinant proteins in E. coli as a fusion protein with neighboring histidine residues is one of the most popular methods of epitope tags. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent.

·         6x-His Epitope Tag Antibody (MA1-21315) in WB

Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315)

Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5ug/mL)

 

·         6x-His Epitope Tag Antibody (MA1-21315) in WB

Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315)

Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2)

 

·         6x-His Epitope Tag Antibody in WB

Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315)

Western blot analysis of His-tagged protein in E. coli lysates. Membranes containing total cell lysates from uninduced E. coli (-) or induced E. coli (+) treated with 1mM Isopropyl beta-D-1-thiogalactopyranoside (IPTG) to induce expression of His-tagged IQ domain (IQD) protein were blocked with 5% non-fat milk in PBS-T and probed with a 6X-His Epitope Tag Antibody (Product # MA1-21315) at a dilution of 1:1400 followed by a donkey anti-mouse IgG-HRP secondary antibody. Blots were developed with SuperSignal West Dura (Product # 34076). His-tagged Repressor of Gibberellin (RGA) was used as a positive control. Data courtesy of the Innovators Program.

 

·         6x-His Epitope Tag Antibody  in IF

          

 

LEFT: untransfected control                RIGHT: anti-His (in red) 

on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)

 

Storage:

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Form:

100 µg of Protein A purified antibody (1 mg/ml) in 10 mM PBS, pH 7.2.


应用类型

WB1:1000 - 1:10000, ICC/IF1:200 - 1:800, IP1:200 - 1:800

免疫原

6x His合成多肽

来源宿主

小鼠

反应性

His标签抗体可识别His重组蛋白和在细胞内过表达的His标记重组蛋白

保存建议

在发货后能在-20℃保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融,分装后可在4℃保存3个月

   
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